Purification and characterization of heat-stable enterotoxin from bovine enterotoxigenic Escherichia coli
نویسندگان
چکیده
منابع مشابه
Purification of heat-labile enterotoxin from an enterotoxin from an enterotoxigenic Escherichia coli of human origin by monoclonal immunoaffinity chromatography.
Heat-labile enterotoxin (LT) was purified from an enterotoxigenic Escherichia coli 015H11 of human origin. The purification steps included French pressure cell disruption of the bacteria, salting-out, DEAE-Sephacel on chromatography. Application of this procedure resulted in a 95.1-fold purification of LT with a yield of 19.9% as determined by rabbit ileal loop assay. The final LT preparation s...
متن کاملProduction and purification of heat-stable enterotoxin b from a porcine Escherichia coli strain.
Production of heat-stable enterotoxin b (STb) by porcine Escherichia coli strains belonging to serogroup O115 was evaluated in ligated intestinal segments of adult rats. The conditions for optimal production and detection of STb were studied by using the STb-producing strain 4247. As STb production was similar in complex Trypticase soy broth and minimal Davis medium, the latter was used for the...
متن کاملComparison of three assay systems for detection of enterotoxigenic Escherichia coli heat-stable enterotoxin.
In this study, a commercial DNA-DNA hybridization kit for the detection of Escherichia coli heat-stable enterotoxin is compared with a competitive enzyme-linked immunosorbent assay (ELISA) and the suckling mouse bioassay. Taking the suckling mouse assay as the "gold standard," the gene probe was the more specific and the ELISA was the more sensitive of the assays. The ELISA and the suckling mou...
متن کاملHeat-stable enterotoxin of enterotoxigenic Escherichia coli as a vaccine target.
Enterotoxigenic Escherichia coli (ETEC) is responsible for 280 million to 400 million episodes of diarrhea and about 380,000 deaths annually. Epidemiological data suggest that ETEC strains which secrete heat-stable toxin (ST), alone or in combination with heat-labile toxin (LT), induce the most severe disease among children in developing countries. This makes ST an attractive target for inclusi...
متن کاملCharacterization of Escherichia coli strains producing heat-stable enterotoxin b (STb) isolated from humans with diarrhea.
Two of 49 cytolethal distending toxin-producing strains of Escherichia coli isolated from human stools contained the gene coding for heat-stable enterotoxin b (STb), as detected by a colony hybridization assay. The STb gene was found to be on a 70-kb plasmid also coding for heat-labile enterotoxin (pLT-I). Restriction endonuclease analysis showed the STb gene from human isolates to be similar t...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1983
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.40.2.701-707.1983